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AD7c-NTP Rabbit pAb

AD7c-NTP Rabbit pAb

     
  •  - AD7c-NTP Rabbit pAb AP94710
    Blank control(black line):U87MG. Primary Antibody (green line): Rabbit Anti-AD7c-NTP antibody (AP94710) Dilution:1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Negative control(white blue line): PBS Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
  • WB - AD7c-NTP Rabbit pAb AP94710
    Sample: AD7c-NTP recombinant protein(Human) Lysate at 100ng Primary: Anti-AD7c-NTP(AP94710)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 41kD Observed band size: 46kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Reactivity Human
Host Rabbit
Clonality Polyclonal
Additional Information
Dilution WB=1:500-2000,Flow-Cyt=1ug/Test
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Citations (0)
citation

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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94710
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