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>   home   >   Products   >   Secondary Antibodies   >   ATTO   >   Anti-Rabbit IgG (H&L) (ATTO 425 Conjugated) Pre-Adsorbed Secondary Antibody   

Anti-Rabbit IgG (H&L) (ATTO 425 Conjugated) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, ATTO 425

     
  • IF - Anti-Rabbit IgG (H&L)  (ATTO 425 Conjugated) Pre-Adsorbed Secondary Antibody ASR1165
    ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells) stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
    detail
  • WB - Anti-Rabbit IgG (H&L)  (ATTO 425 Conjugated) Pre-Adsorbed Secondary Antibody ASR1165
    Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
    detail
  • WB - Anti-Rabbit IgG (H&L)  (ATTO 425 Conjugated) Pre-Adsorbed Secondary Antibody ASR1165
    Western Blot of ATTO 425 conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 425 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa/55 kDa for Rabbit IgG. Other band(s): none.
    detail
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Product Information
Description Anti-RABBIT IgG (H&L) (GOAT) Antibody ATTO 425 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
Host Goat
Conjugate ATTO 425
FP Value 2.3 moles ATTO 425 per mole of IgG
Target Species Rabbit
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Application Note FLISA >1:20,000
IF Microscopy >1:5,000
Western Blot >1:10,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rabbit IgG whole molecule
Reconstitution Volume 100 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity Rabbit IgG (H&L) Antibody ATTO 425 was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
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Background

Anti-Rabbit IgG (H&L) conjugated to ATTO 425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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$ 99.00
Cat# ASR1165
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