KD-Validated Anti-GBE1 Mouse Monoclonal Antibody

Western blotting analysis using anti-GBE1 antibody (Cat#AGI2056). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GBE1 antibody (Cat#AGI2056, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
Western blotting analysis using anti-GBE1 antibody (Cat#AGI2056). GBE1 expression in wild-type (WT) and GBE1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-GBE1 antibody (Cat#AGI2056, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.

SPECIFICATION
CITATIONS
PROTOCOLS
BACKGROUND

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB
Primary Accession	Q04446
Reactivity	Human
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone Names	24GB13015
Calculated MW	Predicted, 80 kDa, observed, 70 kDa
Gene Name	GBE1
Aliases	GBE1; 1,4-Alpha-Glucan Branching Enzyme 1; Glucan (1,4-Alpha-), Branching Enzyme 1; 1,4-Alpha-Glucan-Branching Enzyme; Glycogen Branching Enzyme; Brancher Enzyme; EC 2.4.1.18; Amylo-(1,4 To 1,6) Transglucosidase; Amylo-(1,4 To 1,6) Transglycosylase; Glycogen Storage Disease Type IV; Glycogen-Branching Enzyme; Andersen Disease; APBD; GSD4; GBE
Immunogen	Recombinant protein of human GBE1

Additional Information
Gene ID	2632
Other Names	1, 4-alpha-glucan-branching enzyme, 2.4.1.18, Brancher enzyme, Glycogen-branching enzyme, GBE1

Protein Information
Name	GBE1
Function	Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer (PubMed:26199317, PubMed:8463281, PubMed:8613547).

Research Areas

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Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

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Mouse monoclonal antibody

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