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Anti-Alpha-Adaptin Antibody (Monoclonal, 100/2)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | D3ZUY8 |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for alpha-Adaptin, adaptor-related protein complex 2, alpha 1 subunit (AP2A1 ) detection. Tested with WB in Human;rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Calculated MW | 107675 MW KDa |
|---|---|
| Application Details | Western blot, 2-4 µg/ml, Human, rat |
| Subcellular Localization | AP2A1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.|Ap2a1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV. |
| Tissue Specificity | AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells. |
| Protein Name | AP-2 complex subunit alpha-1 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | 100/2 |
| Immunogen | AP-2 adaptor polypeptides from bovine brain. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the adaptor complexes large subunit family. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al.(2001) identified 3 HIP1-associated proteins, clathrin heavy chain(CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD(adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
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