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TPPP/p25 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - TPPP/p25 Antibody (internal region) AF3088a
    AF3088a (0.001 µg/ml) staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - TPPP/p25 Antibody (internal region) AF3088a
    AF3088a (3.8 µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O94811
Other Accession NP_008961.1, 11076, 72948 (mouse), 361466 (rat)
Reactivity Human, Mouse, Rat
Predicted Pig
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 23694 Da
Additional Information
Gene ID 11076
Other Names Tubulin polymerization-promoting protein, TPPP, 25 kDa brain-specific protein, TPPP/p25, p24, p25-alpha, TPPP, TPPP1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPPP/p25 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPPP {ECO:0000303|PubMed:17105200, ECO:0000312|HGNC:HGNC:24164}
Function Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed:31522887). Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed:31522887). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed:31522887). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (PubMed:21995432, PubMed:21316364). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed:17105200, PubMed:17693641, PubMed:18028908, PubMed:26289831). Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6 (PubMed:20308065, PubMed:23093407). Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in increased acetylation of tubulin and increased cell motility (PubMed:23093407). Plays a role in cell proliferation by regulating the G1/S-phase transition (PubMed:23355470). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (PubMed:22328514).
Cellular Location Postsynaptic Golgi apparatus {ECO:0000250|UniProtKB:D3ZQL7}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000250|UniProtKB:D3ZQL7}. Cytoplasm, cytoskeleton. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, which shapes dendrite morphology by functioning as sites of acentrosomal microtubule nucleation (By similarity). Mainly localizes to the cytoskeleton (PubMed:18028908). Also found in the nucleus; however, nuclear localization is unclear and requires additional evidences (PubMed:18028908). Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies (PubMed:15590652, PubMed:17027006) During mitosis, colocalizes with LIMK2 at the mitotic spindle (PubMed:22328514). {ECO:0000250|UniProtKB:D3ZQL7, ECO:0000269|PubMed:15590652, ECO:0000269|PubMed:17027006, ECO:0000269|PubMed:18028908, ECO:0000269|PubMed:22328514}
Tissue Location Widely expressed..
Research Areas
Citations (0)

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An unstructured protein with destructive potential: TPPP/p25 in neurodegeneration. Ovádi J, Orosz F. BioEssays : news and reviews in molecular, cellular and developmental biology 2009 Jun 31 (6): 676-86. PMID: 19382230

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$ 368.00
Cat# AF3088a
Availability: 7-10 days
Bulk Size

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