PARD3 Antibody (aa1141-1190)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
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Primary Accession | Q8TEW0 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 151kDa |
Dilution | ELISA (1:20000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) , |
Gene ID | 56288 |
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Other Names | Partitioning defective 3 homolog, PAR-3, PARD-3, Atypical PKC isotype-specific-interacting protein, ASIP, CTCL tumor antigen se2-5, PAR3-alpha, PARD3, PAR3, PAR3A |
Target/Specificity | PARD3 Antibody detects endogenous levels of total PARD3 protein. |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | PARD3 Antibody (aa1141-1190) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PARD3 (HGNC:16051) |
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Synonyms | PAR3, PAR3A |
Function | Adapter protein involved in asymmetrical cell division and cell polarization processes (PubMed:27925688, PubMed:10954424). Seems to play a central role in the formation of epithelial tight junctions (PubMed:27925688). Targets the phosphatase PTEN to cell junctions (By similarity). Involved in Schwann cell peripheral myelination (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (By similarity). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:10934474). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (PubMed:19812038, PubMed:27925688). |
Cellular Location | Cytoplasm. Endomembrane system. Cell junction. Cell junction, tight junction. Cell junction, adherens junction {ECO:0000250|UniProtKB:Q99NH2}. Cell membrane. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II. Colocalized with SIRT2 in internode region of myelin sheath (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. |
Tissue Location | Widely expressed.. |
Volume | 50 µl |
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Background
Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
References
Joberty G.,et al.Nat. Cell Biol. 2:531-539(2000).
Fang C.M.,et al.Cell Res. 11:223-229(2001).
Kohjima M.,et al.Biochem. Biophys. Res. Commun. 299:641-646(2002).
Gao L.,et al.Gene 294:99-107(2002).
Deloukas P.,et al.Nature 429:375-381(2004).
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