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Phospho-PLM(S68) Antibody
Purified Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9Z239 |
| Reactivity | Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone/Animal Names | 41AT858.235.86 |
| Calculated MW | 10323 Da |
| Gene ID | 56188 |
|---|---|
| Other Names | Phospholemman, FXYD domain-containing ion transport regulator 1, Fxyd1, Plm |
| Target/Specificity | This PLM Antibody is generated from mice immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S68 of mouse PLM. |
| Dilution | WB~~1:100~500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-PLM(S68) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Fxyd1 {ECO:0000312|MGI:MGI:1889273} |
|---|---|
| Function | Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell (PubMed:15563542, PubMed:18065526). Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated (By similarity). Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation- mediated inhibition of ATP1B1 (PubMed:21454534). Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone (PubMed:19187398). |
| Cellular Location | Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P56513}; Single-pass type I membrane protein. Apical cell membrane {ECO:0000250|UniProtKB:O08589}; Single-pass type I membrane protein. Membrane, caveola {ECO:0000250|UniProtKB:O08589}. Cell membrane, sarcolemma, T-tubule {ECO:0000250|UniProtKB:O08589}. Note=Detected in the apical cell membrane in brain. In myocytes, localizes to sarcolemma, t-tubules and intercalated disks. {ECO:0000250|UniProtKB:O08589} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the FXYD family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity and act as an accessory protein of Na,K-ATPase. Alternatively spliced transcript variants have been described.
References
Phospholemman and beta-adrenergic stimulation in the heart. Wang J, et al. Am J Physiol Heart Circ Physiol, 2010 Mar. PMID 20008271.
Extracellular potassium dependence of the Na+-K+-ATPase in cardiac myocytes: isoform specificity and effect of phospholemman. Han F, et al. Am J Physiol Cell Physiol, 2009 Sep. PMID 19570895.
FXYD1, a modulator of Na,K-ATPase activity, facilitates female sexual development by maintaining gonadotrophin-releasing hormone neuronal excitability. Garcia-Rudaz C, et al. J Neuroendocrinol, 2009 Feb. PMID 19187398.
Cell volume control in phospholemman (PLM) knockout mice: do cardiac myocytes demonstrate a regulatory volume decrease and is this influenced by deletion of PLM? Bell JR, et al. Exp Physiol, 2009 Mar. PMID 19074587.
Regulation of cardiac myocyte contractility by phospholemman: Na+/Ca2+ exchange versus Na+ -K+ -ATPase. Song J, et al. Am J Physiol Heart Circ Physiol, 2008 Oct. PMID 18708446.
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