|Application ||IF, IHC-P, WB, E|
|Calculated MW||76614 Da|
|Other Names||Nucleolin, Protein C23, NCL|
|Target/Specificity||This NUCL Monoclonal antibody is generated from mouses immunized with a protein from human NUCL.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NUCL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.|
|Cellular Location||Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs|
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Provided below are standard protocols that you may find useful for product applications.
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010) Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010) Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010) Bertrand, L., et al. J. Virol. 84(1):109-118(2010) Jerke, U., et al. PLoS ONE 4 (12), E8302 (2009)
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