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AFP Antibody (N-term)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P02771 |
| Other Accession | NP_001125.1 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgM |
| Clone/Animal Names | 143CT50.11.8 |
| Calculated MW | 68678 Da |
| Antigen Region | 56-85 aa |
| Gene ID | 174 |
|---|---|
| Other Names | Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP |
| Target/Specificity | This AFP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human AFP. |
| Dilution | WB~~1:31.25 E~~Use at an assay dependent concentration. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AFP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AFP |
|---|---|
| Synonyms | HPAFP |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| Cellular Location | Secreted. |
| Tissue Location | Plasma. Synthesized by the fetal liver and yolk sac |
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Provided below are standard protocols that you may find useful for product applications.
Background
alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
References
Suriapranata, I.M., et al. Clin. Chim. Acta 411 (5-6), 351-358 (2010)
Vibert, E., et al. Am. J. Transplant. 10(1):129-137(2010)
Potapovich, A.I., et al. Br. J. Pharmacol. 158(5):1236-1247(2009)
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