|Application ||WB, E|
|Calculated MW||24824 Da|
|Other Names||Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1|
|Target/Specificity||This UCHL1 monoclonal antibody is generated from mouse immunized with UCHL1 recombinant protein.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UCHL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865).|
|Cellular Location||Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain|
|Tissue Location||Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)
Hussain, S., et al. Leukemia 24(9):1641-1655(2010)
Ma, Y., et al. J. Cell. Biochem. 110(6):1512-1519(2010)
Wu, Y.R., et al. Clin. Chim. Acta 411 (13-14), 955-958 (2010) :
Li, L., et al. Clin. Cancer Res. 16(11):2949-2958(2010)
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