|Application ||WB, E|
|Calculated MW||46939 Da|
|Other Names||[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, Pyruvate dehydrogenase kinase isoform 3, PDK3, PDHK3|
|Target/Specificity||Purified His-tagged PDK3 protein(Fragment) was used to produced this monoclonal antibody.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDK3 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.|
|Cellular Location||Mitochondrion matrix.|
|Tissue Location||Expressed in heart, skeletal muscle, spinal cord, as well as fetal and adult brain|
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Provided below are standard protocols that you may find useful for product applications.
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Lu, C.W., et al. J. Biol. Chem. 283(42):28106-28114(2008)
Degenhardt, T., et al. J. Mol. Biol. 372(2):341-355(2007)
Kato, M., et al. Structure 15(8):992-1004(2007)
Devedjiev, Y., et al. J. Mol. Biol. 370(3):407-416(2007)
Tso, S.C., et al. J. Biol. Chem. 281(37):27197-27204(2006)
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