|Application ||WB, E|
|Other Accession||P79338, P03180, NP_000563.1|
|Calculated MW||20517 Da|
|Antigen Region||27-53 aa|
|Other Names||Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10|
|Target/Specificity||This IL10 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 27-53 amino acids from human IL10.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IL10 Antibody(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2- mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen- presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).|
|Tissue Location||Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq].
Huebinger, R.M., et al. J. Surg. Res. 164 (1), E141-E145 (2010) :
Glocker, E.O., et al. Lancet 376 (9748), 1272 (2010) :
Mosaad, Y.M., et al. Scand. J. Immunol. 72(4):358-364(2010)
Kung, W.J., et al. Diabetes Technol. Ther. 12(10):809-813(2010)
Santhosh, S., et al. Trop Gastroenterol 31(1):30-33(2010)
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