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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD33 Antibody   

CD33 Antibody

Mouse Monoclonal Antibody (Mab)

     
  • IF - CD33 Antibody AM2149b
    Fluorescent image of A549 cell stained with CD33 Antibody(Cat#AM2149b/SM120504AC).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CD33 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃. Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).CD33 immunoreactivity is localized to Membrane significantly.
  • WB - CD33 Antibody AM2149b
    CD33 Antibody(Cat. #AM2149b) western blot analysis in HepG2 cell line lysates (35μg/lane).This demonstrates the CD33 antibody detected the CD33 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession P20138
Other Accession NP_001763.3
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names 574CT16.4.4
Calculated MW 39825 Da
Antigen Region 337-364 aa
Additional Information
Gene ID 945
Other Names Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33, CD33, SIGLEC3
Target/Specificity This CD33 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 337-364 amino acids from human CD33.
Dilution IF~~1:10~50
WB~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD33 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD33
Synonyms SIGLEC3
Function Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid- bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine- based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein- tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).
Cellular Location Isoform CD33M: Cell membrane; Single-pass type I membrane protein
Tissue Location Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells
Research Areas
Citations (0)

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Background

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Akahane, K., et al. Leukemia 24(4):865-869(2010)
Shamsasenjan, K., et al. Int. J. Hematol. 89(3):310-318(2009)
Bertram, L., et al. Am. J. Hum. Genet. 83(5):623-632(2008)

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$ 325.00
$ 109.00
Cat# AM2149b
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