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ABO Antibody (N-term) (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • WB - ABO Antibody (N-term) (Ascites) AM2158a
    ABO Antibody (N-term)(Ascites)(Cat. #AM2158a) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the ABO antibody detected the ABO protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P16442
Other Accession NP_065202.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone/Animal Names 593CT3.2.2
Calculated MW 40934 Da
Antigen Region 41-68 aa
Additional Information
Gene ID 28
Other Names Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, A transferase, Histo-blood group B transferase, B transferase, NAGAT, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form, ABO
Target/Specificity This ABO antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 41-68 amino acids from the N-terminal region of human ABO.
Dilution WB~~1:200~6400
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABO Antibody (N-term) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABO
Function This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
Cellular Location Golgi apparatus, Golgi stack membrane; Single- pass type II membrane protein. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid
Research Areas
Citations (0)
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Background

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene.

References

Thuresson, B., et al. Transfusion 48(3):493-504(2008)
Seltsam, A., et al. Transfusion 47(12):2330-2335(2007)
Twu, Y.C., et al. Transfusion 46(11):1988-1996(2006)
Jenkins, P.V., et al. Transfusion 46(10):1836-1844(2006)
Roubinet, F., et al. Transfusion 44(5):707-715(2004)

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$ 182.50
Cat# AM2158a
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