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Phospho-Tyr15 cdc2 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Tyr15 cdc2 Antibody AN1004
    Western blot of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: λ-Ptase). These blots are identical to the control except that they were incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P39951
Reactivity Human, Xenopus
Predicted Mouse, Rat, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 34 KDa
Additional Information
Gene ID 54237
Gene Name CDK1
Other Names Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, Cdk1, Cdc2, Cdc2a, Cdkn1
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the λ-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Tyr15 cdc2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).


Dranovsky A, Vincent I, Gregori L, Schwarzman A, Colflesh D, Enghild J, Strittmatter W, Davies P, Goldgaber D (2001) cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer's disease pathology. Neurobiol Aging 22:517-528.
Konishi Y, Bonni A (2003) The E2F-cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons. J Neurosci 23:1649-1658.
Maller JL (1991) Mitotic control. Curr Opin Cell Biol 3:269-275.
Nakamizo A, Inamura T, Inoha S, Amano T, Ochi H, Ikezaki K, Fukui M (2002) Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines. J Neurooncol 59:7-13.
Okano HJ, Pfaff DW, Gibbs RB (1996) Expression of EGFR-, p75NGFR-, and PSTAIR (cdc2)-like immunoreactivity by proliferating cells in the adult rat hippocampal formation and forebrain. Dev Neurosci 18:199-209.

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$ 365.00
Cat# AN1004
Availability: 7-10 days
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