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Phospho-Ser294 Progesterone Receptor Antibody

Affinity purified mouse monoclonal antibody

  • WB - Phospho-Ser294 Progesterone Receptor  Antibody AN1023
    Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06401
Reactivity Human
Predicted Monkey
Host mouse
Clonality monoclonal
Isotype IgG1
Clone Names 608
Calculated MW 90/120 KDa
Additional Information
Gene ID 5241
Gene Name PGR
Other Names Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared by affinity purification using a protein G column.
Antibody Specificity Specific for the ~90k PR-A isoform and the ~120k PR-B isoformphosphorylated at Ser294. Immunolabeling is blocked by the phosphopeptide used as theantigen but not by the corresponding dephosphopeptide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser294 Progesterone Receptor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
Citations (0)

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There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser 190 and Ser 294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.


Attia GR, Zeitoun K, Edwards D, Johns A, Carr BR, Bulun SE (2000) Progesterone receptor isoform A but not B is expressed in
endometriosis. J Clin Endocrinol Metab 85:2897-2902.
Clemm DL, Sherman L, Boonyara
tanakornkit V, Schrader WT, Weigel NL, Edwa
rds DP (2000) Differential hormone-dependent
phosphorylation of progesterone receptor A and B forms reveal
ed by a phosphoserine site-specific monoclonal antibody.
Mol Endocrinol 14:52-65.
Edwards DP (2005) Regulation of signal transduction pathwa
ys by estrogen and progesterone. Annu Rev Physiol 67:335-376.
Lin VC, Woon CT, Aw SE, Guo C (2003) Distinct molecular path
ways mediate progesterone-induced growth inhibition and
focal adhesion. Endocrinology 144:5650-5657.

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$ 365.00
Cat# AN1023
Availability: 7-10 days
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