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Tryptophan Hydroxylase Antibody
Affinity purified sheep polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P17290 |
| Reactivity | Human, Rat |
| Host | Sheep |
| Clonality | polyclonal |
| Calculated MW | 55 KDa |
| Gene ID | 100101558 |
|---|---|
| Gene Name | TPH1 |
| Other Names | Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH |
| Target/Specificity | Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from sheep serum by affinity purification using a column to which the recombinant protein was coupled. |
| Antibody Specificity | Specific for the ~55k tryptophan hydroxylase protein. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Tryptophan Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).
References
Haycock JW, Kumer SC, Lewis DA, Vrana KE, Stockmeier CA (2002) A monoclonal antibody to tryptophan
hydroxylase: applications and identification of the epitope. J Neurosci Methods 114:205-212.
Martinez A, Knappskog PM, Haavik J (2001) A structural approach into human tryptophan hydroxylase and its
implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.
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