Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Rabphilin 3A Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P47709 |
| Reactivity | Rat |
| Predicted | Bovine, Human, Mouse, Monkey |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 82 KDa |
| Gene ID | 171039 |
|---|---|
| Gene Name | RPH3A |
| Other Names | Rabphilin-3A, Exophilin-1, Rph3a |
| Target/Specificity | Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification on a column to which the C-terminal peptide immunogen was coupled. |
| Antibody Specificity | Specific for the ~82k rabphilin 3A protein. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Rabphilin 3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Rabphilin-3A is a peripheral membrane protein that binds Ca 2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser 234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).
References
Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of
phosphorabphilin. J Neurosci 21:5461-5472.
Fykse EM, Li C, Sudhof TC (1995) Phosphorylatio
n of rabphilin-3A by Ca2+/calmodulin- and cAMP-
dependent protein kinases in vitro. J Neurosci 15:2385-2395.
Geppert M, Bolshakov VY, Siegelbaum SA, Takei K,
De Camilli P, Hammer RE, Südhof TC (1994) The
role of Rab3A in neurotransmitter release. Nature (Lond ) 369:493-497.
Li C, Takei K, Geppert M, Daniell L, Stenius K,
Chapman ER, Jahn R, De Camilli P, Südhof TC (1994)
Synaptic targeting of rabphilin
-3A, a synaptic vesicle Ca
2+
/phospholipid- binding protein, depends on
rab3A/3C. Neuron 13:885-898.
Smith R, Petersen A, Bates GP, Brundin P, Li JY (20
05) Depletion of rabphilin 3A in a transgenic mouse
model (R6/1) of Huntington's disease, a possible
culprit in synaptic dysfunction. Neurobiol Dis 20:673-
684.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
