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NSF Antibody

Affinity purified rabbit polyclonal antibody

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  • IHC - NSF Antibody AN1110
    Immunohistochemical analysis of paraffin-embedded R. brain section using NSF Antibody (Cat#AN1110). AN1110 was diluted at 1:500 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC - NSF Antibody AN1110
    Immunohistochemical analysis of paraffin-embedded M. brain section using NSF Antibody (Cat#AN1110). AN1110 was diluted at 1:500 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • FC - NSF Antibody AN1110
    Flow cytometric analysis of PC-12 cells using Park7 (DJ-1) Antibody(green, Cat#AN1110) compared to an isotype control of rabbit IgG(blue). AN1110 was diluted at 1:500 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - NSF Antibody AN1110
    Western blot analysis of lysates from mouse C2C12 cell line, rat brain and mouse brain tissue lysate(from left to right), using Nsf Antibody(Cat. #AN1110). AN1110 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - NSF Antibody AN1110
    Western blot of rat caudate lysate showing specific labeling of the~75k NSF protein.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9QUL6
Reactivity Rat
Predicted Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 75 KDa
Additional Information
Gene ID 60355
Gene Name NSF
Other Names Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, Nsf, Erg1
Target/Specificity Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Dilution IHC~~1:500
WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.
Antibody Specificity Specific for ~75k NSF protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNSF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
Citations (0)

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NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).


Schweizer FE, Dresbach T, DeBello WM, O’Connor V, Augustine G, Betz H (1998) Regulation of neurotransmitter release kinetics by NSF. Science 279(5354):1203-1206.
Matveeva EA, Whitehart SW, Vanaman T, Slevin J (2001) Phosphorylation of the N-ethylmaleimide sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes. J Biol.Chem. 276(15): 12174-12181.
Whitehart SW, Matveeva EA (2004) Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor. J Struct. Biol.146(1-2): 32-43.

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$ 365.00
Cat# AN1110
Availability: 7-10 days
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