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Phospho-Ser515 Munc-18 Antibody

Affinity purified rabbit polyclonal antibody

  • IHC - Phospho-Ser515 Munc-18 Antibody AN1116
    Immunohistochemical analysis of paraffin-embedded R. brain section using Phospho-Ser515 Munc-18 Antibody (Cat#AN1116). AN1116 was diluted at 1:400 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • FC - Phospho-Ser515 Munc-18 Antibody AN1116
    Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green, Cat#AN1116) compared to an isotype control of rabbit IgG(blue). AN1116 was diluted at 1:400 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - Phospho-Ser515 Munc-18 Antibody AN1116
    Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide(peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
  • IHC - Phospho-Ser515 Munc-18 Antibody AN1116
    Immunostaining of 14 DIV cortical neurons showing specific labeling of Munc-18 phosphorylated at Ser515 (red) and PSD95 (green).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q62753
Reactivity Rat
Predicted Bovine, Chicken, Human, Mouse, Monkey, Xenopus
Host Rabbit
Clonality polyclonal
Calculated MW 65 KDa
Additional Information
Gene ID 81804
Gene Name STXBP2
Other Names Syntaxin-binding protein 2, Protein unc-18 homolog 2, Munc18-2, Unc18-2, Protein unc-18 homolog B, Unc-18B, Stxbp2, Unc18b
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser515 conjugated to KLH.
Dilution IHC~~ 1:400
WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser515 Munc-18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).


Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482.
Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL (2004) Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo. J Biol Chem 279:55924-55936.
Sassa T, Ogawa H, Kimoto M, Hosono R (1996) The synaptic protein UNC-18 is phosphorylated by protein kinase C. Neurochem Int 29:543-552.
Sergio Leal-Ortiz, Clarissa L. Waites, Ryan Terry-Lorenzo, Pedro Zamorano, Eckart D. Gundelfinger, and Craig C. Garner (2008) Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181: 831 - 846.

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$ 365.00
Cat# AN1116
Availability: 7-10 days
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