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GABA Transporter (GAT) 3 Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - GABA Transporter (GAT) 3 Antibody AN1130
    Western blot of rat hippocampal homogenate showing specificimmunolabeling of the~ 67k GAT-3 protein.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P31647
Reactivity Rat
Predicted Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 67 KDa
Additional Information
Gene ID 79213
Gene Name SLC6A11
Other Names Sodium- and chloride-dependent GABA transporter 3, GAT-3, Solute carrier family 6 member 11, Slc6a11, Gabt3, Gat-3, Gat-b
Target/Specificity Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via chromatography with a column made with the peptide used as antigen.
Antibody Specificity Specific for the ~67k GAT-3 protein. Immunolabeling is blocked by thepeptide used as antigen.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGABA Transporter (GAT) 3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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citation

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Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-3 has been found to be localized to astrocytes within the cerebral cortex indicating that this transporter mediates GABA uptake into glial cells (Minelli et al., 1996).

References

Minelli A, DeBiasi S, Brecha NC, Zuccarello LV, Conti F (1996) GAT-3, a high-affinity GABA plasma
membrane transporter, is localized to astrocytic processes, and is not confined to the vicinity of
GABAergic synapses in the cerebral cortex. J. Neurosci. 16(19):6255-64.

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$ 365.00
Cat# AN1130
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Availability: 7-10 days
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