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Fto (Fat mass and obesity-associated protein) Antibody

Mouse monoclonal antibody

  • WB - Fto (Fat mass and obesity-associated protein) Antibody AN1187
    Western blot of rat testes lysate showing specificimmunolabeling of the ~ 58k Fto protein.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9C0B1
Reactivity Human, Mouse, Rat
Predicted Bovine, Monkey
Host Mouse
Clonality monoclonal
Isotype IgG
Clone Names 5-2H10
Calculated MW 58 KDa
Additional Information
Gene ID 79068
Gene Name FTO
Other Names Alpha-ketoglutarate-dependent dioxygenase FTO, 11411-, Fat mass and obesity-associated protein, FTO, KIAA1752
Target/Specificity Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
Dilution WB~~ 1:1000
Format Protein G purified culture supernatant.
Antibody Specificity Specific for the ~58 kDa Fto protein in Western blots of rat testes lysate.The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFto (Fat mass and obesity-associated protein) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009)


Fredriksson R, Hägglund M, Olszewski PK, Stephansson O, Jacobsson JA, Olszewska AM,
Levine AS, Lindblom J, Schiöth HB (2008). The obesity gene, FTO, is of ancient origin,
upregulated during food deprivation and expressed in neurons of feeding-related nuclei of the
brain. Endocrinology 149: 2062.
Fischer J, Koch L, Emmerling C, Vierkotten J, Peters T, Brüning JC, Rüther U. (2009).
Inactivation of the Fto gene protects from obesity. Nature 458: 894.

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$ 365.00
Cat# AN1187
Availability: 7-10 days
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