|Reactivity||Human, Mouse, Rat|
|Calculated MW||62 KDa|
|Other Names||Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182|
|Target/Specificity||Recombinant full length human COBRA1 purified from E. coli .|
|Format||Protein G purified culture supernatant|
|Antibody Specificity||Specific for the ~62k COBRA1 protein in Western blots of neonatal rat brain lysate.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||COBRA 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004)
Aiyar, SE, Sun J, Blair AL, Moskaluk CA, Lu Y, Ye Q, Yamaguchi Y, Mukherjee A, Ren D, Handa H, Li R (2004). Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18 (17): 2134–46.
Zhong, H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Qi (2004). COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun.325 (2): 568–73.
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