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Phospho-Ser23/24 Troponin I (cardiac) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser23/24 Troponin I (cardiac) Antibody AN1223
    Western blot of mouse heart homogenate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser23/24 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48787
Reactivity Mouse, Rat
Predicted Human, Monkey
Host Rabbit
Clonality polyclonal
Calculated MW 25 KDa
Additional Information
Gene ID 21954
Gene Name TNNI3
Other Names Troponin I, cardiac muscle, Cardiac troponin I, Tnni3
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Antibody Specificity Specific for the ~25k cardiac troponin I protein phosphorylated at Ser23/24.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser23/24 Troponin I (cardiac) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
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Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, and when cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 it causes regulation of Ca2+- stimulated ATPase (Noland et al, 1995). Evidence suggests that phosphorylation of both serines 23 and 24 is required for the reduction in Ca2+-sensitivity and beneficial for relaxation of the heart (Kooij et al, 2010). Ser23/24 phosphorylation is important for enhanced relaxation in response to prolonged activation of protein kinase C by endothelin in intact myocytes, while Thr144 plays an important role in the acute acceleration of relaxation (Westfall et al, 2005).


Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.
Viola Kooij, Martina Saes, Kornelia Jaquet, Ruud Zaremba, D. Brian Foster, Anne M. Murphy, Cris dos Remedios, Joland van der Velden, Ger J.M. Stienen (2010) Effect of Troponin I ser23/24 phosphorylation on Ca2+-sensitivity in human myocardium depends on the phosphorylation background. J of Mol Cell Card 48: 954-963.
Margaret V. Westfall, Adonia M. Lee, and Dustin A. Robinson (2005) Differential Contribution of Troponin I Phosphorylation Sites to

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$ 365.00
Cat# AN1223
Availability: 7-10 days
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