|Application ||FC, WB|
|Predicted||Human, Mouse, Pig, Monkey, Zebrafish|
|Calculated MW||25 KDa|
|Other Names||Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, Snap25, Snap|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~25k SNAP25 protein phosphorylated at Ser187 in Western blots. Immunolabeling is completely blocked by blocked λ-Ptase.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser187 SNAP25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).
Ando K, Kudo Y, Takahashi M (2005) Negative regulation of neurotransmitter release by calpain: a possible specific involvement of Snap25 cleavage. J Neurochem 94(3):651-8.
Fatemi SH, Earle JA, Stary JM, Lee S, Sedgwick J (2001) Altered levels of the synaptosomal associated protein Snap25 in hippocampus of patients with mood disorders and Schizophrenia. Neuroreport. 12(15): 3257-62.
Fatemi SH, Sidwell R, Kist D, Akhter P, Meltzer HY, Bailey K, Thuras P, Sedgwick J (1998) Differential expression of synaptosome-associated protein 25 kDa (Snap25) in hippocampi of neonatal mice following exposure to human influenza virus in utero. Brain Res 800(1):1-9.
Greber S, Cairns N, Lubec G, Fountoulakis M (1999) Decreased levels of the synaptosomal associated protein 25 in the brains of patients with Down Syndrome and Alzheimer’s Disease. Electrophoresis 20 (4-5):928-34.
Pozzi D, Condliffe S, Bozzi Y, Chikhladze M, Grumelli C, Proux-Gillardeaux V, Takahashi M, Franceschetti S, Verderio C, Matteoli M (2008) Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. PNAS 105: 323-328.
Shu Y, Liu X, Yang Y, Takahashi M, Gillis KD (2008) Phosphorylation of SNAP-25 at Ser187 Mediates Enhancement of Exocytosis by a Phorbol Ester in INS-1 Cells. J of Neurosci 28: 21-30.
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