Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Phospho-Thr53 Dopamine Transporter (DAT) Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P23977 |
| Reactivity | Rat |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 55 KDa |
| Gene Name | SLC6A3 |
|---|---|
| Other Names | Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3, Slc6a3 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Antibody Specificity | Specific for the ~55k glycosylated form of the DAT proteinphosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation ofthe DAT protein. Immunolabeling of the DAT band is blocked by preadsorption with thephospho-peptide used as antigen but not by the corresponding dephospho-peptide. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Thr53 Dopamine Transporter (DAT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr53 residue plays a major role in transport activity (Foster et al., 2012).
References
Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock
JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users.
Neuropsychopharmacology 24:561-567.
Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K,
Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic
methamphetamine users. Nat Med 2:699-703.
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the
locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Zhu MY, Klimek V, Dilley GE, Haycock JW, StockmeierC, Overholser JC, Meltzer HY, Ordway GA (1999)
Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry
46:1275-1286.
James D. Foster, Jae-Won Yang, Amy E. Moritz, Sathyavathi ChallaSivaKanaka, Margaret A. Smith,
Marion Holy, Kyle Wilebski, Harald H. Sitte, and Roxanne A. Vaughan (2012) Dopamine Transporter
Phosphorylation Site Threonine 53 Regulates Substrate Reuptake and Amphetamine-stimulated Efflux.
Journal of Biological Chemistry 287(35):29702-29712.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
