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Phospho-Ser43 Troponin I (cardiac) Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P48787 |
| Reactivity | Mouse, Rat |
| Predicted | Bovine, Human, Monkey, Xenopus, Zebrafish |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 25 KDa |
| Gene ID | 21954 |
|---|---|
| Gene Name | TNNI3 |
| Other Names | Troponin I, cardiac muscle, Cardiac troponin I, Tnni3 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. |
| Antibody Specificity | Specific for the ~25k cardiac troponin I protein phosphorylated at Ser43. Immunolabeling is greatly decreased with lambda-phosphatase treatment. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Ser43 Troponin I (cardiac) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKC-mediated depression. (Montgomery et al, 2002).
References
David E. Montgomery, Beata M. Wolska, W. Glen Pyle, Brian B. Roman, Jasmine C. Dowell, Peter M. buttrick, Alan P. Koretsky, Pedro Del Nido, R. John Solaro (2002) α-Adrenergic response and myofilament activity in mouse hearts lacking PKC phosphorylation sites on cardiac TnI. Am J Physiol Heart Circ 282:H2397-H2405.
Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.
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