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Phospho-Thr23/25 REDD1 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Thr23/25 REDD1  Antibody AN1256
    Western blot of human jurkat lysate showing specific immunolabeling of the ~34k REDD1 protein phosphorylated at Thr23/25 (control). Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NX09
Reactivity Human
Host Rabbit
Clonality polyclonal
Calculated MW 34 KDa
Additional Information
Gene ID 54541
Gene Name DDIT4
Other Names DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1, REDD-1, DDIT4, REDD1, RTP801
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 conjugated to KLH.
Dilution WB~~ 1:500
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Antibody Specificity Specific for the ~34k REDD1 phosphorylated at Thr23/25. Immunolabelingis blocked by the phosphopeptide used as antigen but not by the correspondingdephosphopeptide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Thr23/25 REDD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD 1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia c an desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 throu gh the activity of GSK3b (Katiyar et al, 2009).


Schwarzer r, Tondera D, Arnold W, Giese K, Klippel A, Kaufmann J (2005) REDD1 integrates
hypoxia-mediated survival signaling downstream
of phosphatidylinositol 3-kinase. Oncogene
24(7): 1138-49.
Katiyar S, Liu E, Knutzen CA, Lang ES, Lombardo CR, Sankar S, Toth JI, Petroski MD, Ronai
Z, Chiang GC (2009) REDD1, an inhibitor of mTOR signaling, is regulated by the CUL4A-
DDB1 ubiquitin ligase. EMBO Rep 10(8):866-72.

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$ 365.00
Cat# AN1256
Availability: 7-10 days
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