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c-FOS Antibody

Mouse Monoclonal Antibody

     
  • WB - c-FOS Antibody AN1261
    Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein
  • IF - c-FOS Antibody AN1261
    Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear c-FOS labeling of activated cells in green and vimentin in red.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P01100
Reactivity Human, Mouse, Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 2H2
Calculated MW 40695 Da
Additional Information
Gene ID 2353
Gene Name FOS
Target/Specificity Recombinant full length human c-FOS expressed in and purified from E. Coli.
Dilution WB~~ 1:2000
IF~~ 1:1000
Format Protein G purified culture supernatant
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsc-FOS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
Citations (0)

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Background

c-FOS is a member of the FOS transcription factor family which forms dimers with c-JUN to produce the Activator Protein 1 (AP-1) complex which plays a key role in critical cellular processes such as cell proliferation, differentiation and apoptosis (Chiu et al., 1988).  c-FOS expression has been demonstrated to be a useful marker of neuronal activation as it is rapidly induced following various stimuli (Hoffman et al., 1993).  Additionally, c-FOS has been shown to be overexpressed in a variety of malignant tumor types (Milde-Langosch 2005).

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$ 365.00
Cat# AN1261
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