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Microtubule Associated Protein 2 C/D (MAP2C/D) Antibody

Mouse Monoclonal Antibody

     
  • WB - Microtubule Associated Protein 2 C/D (MAP2C/D) Antibody AN1278
    Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70 kDa MAP2C/D protein.
  • IHC - Microtubule Associated Protein 2 C/D (MAP2C/D) Antibody AN1278
    Immunostaining of mixed neuron/glial cultures showing specific cytoplasmic labeling of dendrites and perikarya of neuronal cells in red with anti-MAP2C/D and astrocyte and fibroblast labeling (green) with anti-vimentin 
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P11137
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 2C4
Calculated MW 199526 Da
Additional Information
Gene ID 4133
Gene Name MAP2
Target/Specificity Full length recombinant human MAP2D protein
Dilution WB~~ 1:5000
IHC~~ 1:2500
Format Protein G purified culture supernatant
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMicrotubule Associated Protein 2 C/D (MAP2C/D) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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Background

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa bands referred to as MAP2a and MAP2b. A third lower molecular weight form, MAP2C and MAP2D, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2C and MAP2D are expressed earlier in development than the MAP2a and MAP2b isoforms, so that this antibody is a more useful marker of neuronal development. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (FontaineLenore V. et al., 2006).

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$ 365.00
Cat# AN1278
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