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ALOX12B Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, IHC-P, E |
|---|---|
| Primary Accession | O75342 |
| Other Accession | Q2KMM4, O70582, NP_001130.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 80356 Da |
| Antigen Region | 622-652 aa |
| Gene ID | 242 |
|---|---|
| Other Names | Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase, 11311-, Epidermis-type lipoxygenase 12, ALOX12B |
| Target/Specificity | This ALOX12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 622-652 amino acids from the C-terminal region of human ALOX12B. |
| Dilution | WB~~1:1000 FC~~1:10~50 IHC-P~~1:50~100 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ALOX12B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ALOX12B (HGNC:430) |
|---|---|
| Function | Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:21558561, PubMed:9618483, PubMed:9837935). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561). May also play a role in the regulation of the expression of airway mucins (PubMed:22441738). |
| Cellular Location | Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00726}. Cytoplasm, perinuclear region |
| Tissue Location | Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes an enzyme involved in the converstion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma.
References
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Vahlquist, A., et al. J. Invest. Dermatol. 130(2):438-443(2010)
Agarwal, S., et al. Exp. Dermatol. 18(11):939-946(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)
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