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MRM1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MRM1 Antibody (N-term) AP10383a
    MRM1 Antibody (N-term) (Cat. #AP10383a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MRM1 antibody detected the MRM1 protein (arrow).
    detail
  • IHC-P - MRM1 Antibody (N-term) AP10383a
    MRM1 antibody (N-term) (Cat. #AP10383a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MRM1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - MRM1 Antibody (N-term) AP10383a
    MRM1 Antibody (N-term) (Cat. #AP10383a) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q6IN84
Other Accession NP_079140.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 38638 Da
Antigen Region 80-109 aa
Additional Information
Gene ID 79922
Other Names rRNA methyltransferase 1, mitochondrial, 211-, 16S rRNA (guanosine(1145)-2'-O)-methyltransferase, 16S rRNA [Gm1145] 2'-O-methyltransferase, MRM1 {ECO:0000303|PubMed:24036117}
Target/Specificity This MRM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human MRM1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMRM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MRM1 {ECO:0000303|PubMed:24036117}
Function S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1145 (Gm1145) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA.
Cellular Location Mitochondrion matrix
Research Areas
Citations (0)
citation

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References

Lamesch, P., et al. Genomics 89(3):307-315(2007)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)

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$ 182.50
$ 70.00
Cat# AP10383a
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