|Application ||FC, IHC-P, WB, E|
|Calculated MW||33836 Da|
|Antigen Region||6-33 aa|
|Other Names||Claudin-16, Paracellin-1, PCLN-1, CLDN16, PCLN1|
|Target/Specificity||This CLDN16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-33 amino acids from the N-terminal region of human CLDN16.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CLDN16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors.|
|Cellular Location||Cell junction, tight junction. Cell membrane; Multi-pass membrane protein|
|Tissue Location||Kidney-specific, including the thick ascending limb of Henle (TAL)|
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Provided below are standard protocols that you may find useful for product applications.
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28.
Kuo, S.J., et al. Oncol. Rep. 24(3):759-766(2010)
Efrati, E., et al. Cell. Physiol. Biochem. 25(6):705-714(2010)
Shuen, A.Y., et al. Clin. Chim. Acta 409 (1-2), 28-32 (2009) :
Al-Haggar, M., et al. Clin. Exp. Nephrol. 13(4):288-294(2009)
Lal-Nag, M., et al. Genome Biol. 10 (8), 235 (2009) :
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