DEM1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
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Primary Accession | Q9H790 |
Other Accession | NP_073611.1 |
Reactivity | Human, Hamster, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 41816 Da |
Antigen Region | 166-193 aa |
Gene ID | 64789 |
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Other Names | Exonuclease V, Exo V, hExo5, 31--, Defects in morphology protein 1 homolog, EXO5, C1orf176, DEM1 |
Target/Specificity | This DEM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-193 amino acids from the Central region of human DEM1. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DEM1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EXO5 |
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Synonyms | C1orf176, DEM1 |
Function | Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'- directionality activity. |
Cellular Location | Nucleus. Cytoplasm, cytosol. Note=Localizes to repair foci in response to DNA damage |
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Provided below are standard protocols that you may find useful for product applications.
References
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
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