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KIAA1609 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - KIAA1609 Antibody  (C-term) AP10514b
    Anti-KIAA1609 Antibody (C-term) at 1:8000 dilution + DU145 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - KIAA1609 Antibody  (C-term) AP10514b
    KIAA1609 Antibody (C-term) (Cat. #AP10514b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KIAA1609 antibody detected the K1609 protein (arrow).
    detail
  • IHC-P - KIAA1609 Antibody  (C-term) AP10514b
    KIAA1609 Antibody (C-term) (Cat. #AP10514b) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIAA1609 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - KIAA1609 Antibody  (C-term) AP10514b
    KIAA1609 Antibody (C-term) (Cat. #AP10514b) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q6P9B6
Other Accession NP_065998.3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50994 Da
Antigen Region 426-456 aa
Additional Information
Gene ID 57707
Other Names TLD domain-containing protein 1, TBC/LysM-associated domain-containing protein 1, TLDC1, KIAA1609
Target/Specificity This KIAA1609 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-456 amino acids from the C-terminal region of human K1609.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKIAA1609 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MEAK7 (HGNC:29325)
Synonyms KIAA1609, TLDC1
Function Activates an alternative mTOR signaling through RPS6KB2 activation and EIF4EBP1 repression to regulate cell proliferation and migration (PubMed:29750193). Recruits MTOR at the lysosome, essential for MTOR signaling at the lysosome (PubMed:29750193).
Cellular Location Membrane. Cytoplasm. Lysosome
Research Areas
Citations (0)
citation

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References

Rothenberg, M.E., et al. Nat. Genet. 42(4):289-291(2010)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)

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$ 365.00
$ 140.00
Cat# AP10514b
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