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HIST1H2BG Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HIST1H2BG Antibody (N-term) AP12303a
    Anti-HIST1H2BG Antibody (N-term)at 1:500 dilution + Zebrafish lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P62807
Other Accession P57053, Q9PSW9, P0C1H5, P0C1H4, Q6PC60, Q16778, P0C1H3, P62808, Q8CGP1, Q2PFX4, O60814, Q2M2T1, P06899, Q64478, P10853, Q6ZWY9, P06900, P02281, NP_003513.1, NP_003509.1, NP_003516.1, NP_003517.2, NP_003514.2
Reactivity Human, Zebrafish
Predicted Xenopus, Mouse, Bovine, Monkey, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 13906 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 3017;8339;8343;8344;8346;8347
Other Names Histone H2B type 1-C/E/F/G/I, Histone H2B1 A, Histone H2Ba, H2B/a, Histone H2Bg, H2B/g, Histone H2Bh, H2B/h, Histone H2Bk, H2B/k, Histone H2Bl, H2B/l, HIST1H2BC, H2BFL
Target/Specificity This HIST1H2BG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BG.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H2BG Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2BC4 (HGNC:4757)
Synonyms H2BFL, HIST1H2BC
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)
citation

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].

References

Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
Beck, H.C., et al. Mol. Cell Proteomics 5(7):1314-1325(2006)
Pavri, R., et al. Cell 125(4):703-717(2006)
Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)
Zhu, B., et al. Mol. Cell 20(4):601-611(2005)

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$ 365.00
$ 140.00
Cat# AP12303a
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