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VSGP/F-spondin Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - VSGP/F-spondin Antibody (N-term) AP12414a
    All lanes : Anti-VSGP/F-spondin Antibody (N-term) at 1:2000 dilution Lane 1: Human heart lysate Lane 2: Human lung lysate Lane 3: Mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - VSGP/F-spondin Antibody (N-term) AP12414a
    VSGP/F-spondin Antibody (N-term) (Cat. #AP12414a) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the VSGP/F-spondin antibody detected the VSGP/F-spondin protein (arrow).
  • IHC-P - VSGP/F-spondin Antibody (N-term) AP12414a
    VSGP/F-spondin Antibody (N-term) (Cat. #AP12414a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VSGP/F-spondin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HCB6
Other Accession Q9GLX9, NP_006099.2
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 15-43 aa
Additional Information
Other Names Spondin-1, F-spondin, Vascular smooth muscle cell growth-promoting factor, SPON1, KIAA0762, VSGP
Target/Specificity This VSGP/F-spondin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human VSGP/F-spondin.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVSGP/F-spondin Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPON1
Synonyms KIAA0762, VSGP
Function Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen EMBL; AB051390; BAB18461.1; -; mRNA EMBL; AK074803; BAC11217.1; -; mRNA EMBL; AK291780; BAF84469.1; -; mRNA EMBL; CH471064; EAW68488.1; -; Genomic_DNA EMBL; CH471064; EAW68489.1; -; Genomic_DNA EMBL; BC019825; AAH19825.1; -; mRNA EMBL; BC136513; AAI36514.1; -; mRNA EMBL; BC136563; AAI36564.1; -; mRNA EMBL; AB018305; BAA34482.2; -; mRNA CCDS; CCDS73262.1; - RefSeq; NP_006099.2; NM_006108.3 PDB; 2ZOT; X-ray; 2.70 A; A/B/C/D=29-198 PDB; 2ZOU; X-ray; 1.45 A; A/B=40-186 PDB; 3COO; X-ray; 2.00 A; A/B=29-194 PDB; 3Q13; X-ray; 1.95 A; A=191-434 PDBsum; 2ZOT; - PDBsum; 2ZOU; - PDBsum; 3COO; - PDBsum; 3Q13; - SMR; Q9HCB6; - BioGrid; 115687; 15 IntAct; Q9HCB6; 7 STRING; 9606.ENSP00000460236; - GlyConnect; 1768; - iPTMnet; Q9HCB6; - PhosphoSitePlus; Q9HCB6; - BioMuta; SPON1; - DMDM; 52783472; - EPD; Q9HCB6; - jPOST; Q9HCB6; - MassIVE; Q9HCB6; - PeptideAtlas; Q9HCB6; - PRIDE; Q9HCB6; - ProteomicsDB; 81664; - Antibodypedia; 62004; 156 antibodies Ensembl; ENST00000576479; ENSP00000460236; ENSG00000262655 GeneID; 10418; - KEGG; hsa:10418; - UCSC; uc031xfv.2; human CTD; 10418; - DisGeNET; 10418; - GeneCards; SPON1; - HGNC; HGNC:11252; SPON1 HPA; ENSG00000262655; Tissue enhanced (gallbladder) MIM; 604989; gene neXtProt; NX_Q9HCB6; - OpenTargets; ENSG00000262655; - PharmGKB; PA36082; - GeneTree; ENSGT00940000154910; - HOGENOM; CLU_014540_1_0_1; - InParanoid; Q9HCB6; - OMA; HRMIKMT; - OrthoDB; 517669at2759; - PhylomeDB; Q9HCB6; - Reactome; R-HSA-5083635; Defective B3GALTL causes Peters-plus syndrome (PpS) Reactome; R-HSA-5173214; O-glycosylation of TSR domain-containing proteins ChiTaRS; SPON1; human EvolutionaryTrace; Q9HCB6; - GeneWiki; Spondin_1; - GenomeRNAi; 10418; - Pharos; Q9HCB6; Tbio PRO; PR:Q9HCB6; - Proteomes; UP000005640; Chromosome 11 RNAct; Q9HCB6; protein Bgee; ENSG00000262655; Expressed in female reproductive system and 224 other tissues Genevisible; Q9HCB6; HS GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome GO; GO:0031012; C:extracellular matrix; IBA:GO_Central GO; GO:0005615; C:extracellular space; HDA:BHF-UCL GO; GO:0005201; F:extracellular matrix structural constituent; ISS:BHF-UCL GO; GO:0050693; F:LBD domain binding; IEA:Ensembl GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0007155; P:cell adhesion; IBA:GO_Central GO; GO:1902430; P:negative regulation of amyloid-beta formation; IEA:Ensembl GO; GO:1902993; P:positive regulation of amyloid precursor protein catabolic process; IEA:Ensembl GO; GO:0032092; P:positive regulation of protein binding; IEA:Ensembl GO; GO:0010954; P:positive regulation of protein processing; IEA:Ensembl CDD; cd08544; Reeler; 1 Gene3D;; -; 6 Gene3D;; -; 2 Gene3D;; -; 1 InterPro; IPR002861; Reeler_dom InterPro; IPR042307; Reeler_sf InterPro; IPR009465; Spondin_N InterPro; IPR038678; Spondin_N_sf InterPro; IPR000884; TSP1_rpt InterPro; IPR036383; TSP1_rpt_sf Pfam; PF02014; Reeler; 1 Pfam; PF06468; Spond_N; 1 Pfam; PF00090; TSP_1; 6 SMART; SM00209; TSP1; 6 SUPFAM; SSF82895; SSF82895; 6 PROSITE; PS51019; REELIN; 1 PROSITE; PS51020; SPONDIN; 1 PROSITE; PS50092; TSP1; 6 1: Evidence at protein level; 3D-structure; Cell adhesion; Disulfide bond; Extracellular matrix; Glycoprotein; Metal-binding; Reference proteome; Repeat; Secreted; Signal SIGNAL 1..28 /evidence="ECO:0000255" CHAIN 29..807 /note="Spondin-1" /id="PRO_0000035865" DOMAIN 29..194 /note="Reelin" /evidence="ECO:0000255|PROSITE-ProRule:PRU00363" DOMAIN 195..388 /note="Spondin" /evidence="ECO:0000255|PROSITE-ProRule:PRU00364" DOMAIN 442..495 /note="TSP type-1 1" /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DOMAIN 501..555 /note="TSP type-1 2" /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DOMAIN 558..611 /note="TSP type-1 3" /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DOMAIN 614..666 /note="TSP type-1 4" /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DOMAIN 668..721 /note="TSP type-1 5" /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DOMAIN 754..806 /note="TSP type-1 6" /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" METAL 325 /note="Calcium" /evidence="ECO:0000244|PDB:3Q13, ECO:0000269|PubMed:21569239" METAL 354 /note="Calcium" /evidence="ECO:0000244|PDB:3Q13, ECO:0000269|PubMed:21569239" METAL 358 /note="Calcium; via carbonyl oxygen" /evidence="ECO:0000244|PDB:3Q13, ECO:0000269|PubMed:21569239" CARBOHYD 214 /note="N-linked (GlcNAc...) asparagine" /evidence="ECO:0000244|PDB:3Q13, ECO:0000269|PubMed:21569239" CARBOHYD 448 /note="C-linked (Man) tryptophan" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 451 /note="C-linked (Man) tryptophan; partial" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 507 /note="C-linked (Man) tryptophan" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 510 /note="C-linked (Man) tryptophan; partial" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 564 /note="C-linked (Man) tryptophan" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 620 /note="C-linked (Man) tryptophan; partial" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 623 /note="C-linked (Man) tryptophan" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 674 /note="C-linked (Man) tryptophan" /evidence="ECO:0000269|PubMed:12096136" CARBOHYD 681 /note="N-linked (GlcNAc...) asparagine" /evidence="ECO:0000255" DISULFID 44..128 /evidence="ECO:0000244|PDB:2ZOT, ECO:0000244|PDB:2ZOU, ECO:0000244|PDB:3COO, ECO:0000269|PubMed:18602404, ECO:0000269|PubMed:19020352" DISULFID 156..182 /evidence="ECO:0000244|PDB:2ZOT, ECO:0000244|PDB:2ZOU, ECO:0000244|PDB:3COO, ECO:0000269|PubMed:18602404, ECO:0000269|PubMed:19020352" DISULFID 199..336 /evidence="ECO:0000244|PDB:3Q13, ECO:0000269|PubMed:21569239" DISULFID 200..340 /evidence="ECO:0000244|PDB:3Q13, ECO:0000269|PubMed:21569239" DISULFID 202..415 /evidence="ECO:0000244|PDB:3Q13, ECO:0000269|PubMed:21569239" DISULFID 443..480 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 454..489 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 459..494 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 502..538 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 513..517 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 548..554 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 559..595 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 570..574 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 605..610 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 615..650 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 626..630 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" DISULFID 660..665 /evidence="ECO:0000255|PROSITE-ProRule:PRU00210" CONFLICT 500 /note="S -> P (in Ref. 2; BAC11217)" /evidence="ECO:0000305" CONFLICT 545 /note="T -> M (in Ref. 1; BAB18461)" /evidence="ECO:0000305" CONFLICT 570 /note="C -> Y (in Ref. 2; BAC11217)" /evidence="ECO:0000305" CONFLICT 605 /note="C -> R (in Ref. 2; BAC11217)" /evidence="ECO:0000305" CONFLICT 695 /note="I -> T (in Ref. 4; AAH19825)" /evidence="ECO:0000305" CONFLICT 729 /note="L -> P (in Ref. 1; BAB18461)" /evidence="ECO:0000305" TURN 43..46 /evidence="ECO:0000244|PDB:3COO" TURN 48..50 /evidence="ECO:0000244|PDB:2ZOU" STRAND 60..65 /evidence="ECO:0000244|PDB:2ZOU" STRAND 70..72 /evidence="ECO:0000244|PDB:2ZOU" STRAND 77..84 /evidence="ECO:0000244|PDB:2ZOU" STRAND 90..99 /evidence="ECO:0000244|PDB:2ZOU" STRAND 104..106 /evidence="ECO:0000244|PDB:3COO" HELIX 107..109 /evidence="ECO:0000244|PDB:2ZOU" STRAND 112..117 /evidence="ECO:0000244|PDB:2ZOU" TURN 119..121 /evidence="ECO:0000244|PDB:2ZOU" STRAND 122..125 /evidence="ECO:0000244|PDB:2ZOU" STRAND 128..136 /evidence="ECO:0000244|PDB:2ZOU" STRAND 140..148 /evidence="ECO:0000244|PDB:2ZOU" STRAND 157..169 /evidence="ECO:0000244|PDB:2ZOU" STRAND 173..175 /evidence="ECO:0000244|PDB:2ZOU" STRAND 178..181 /evidence="ECO:0000244|PDB:2ZOU" STRAND 203..213 /evidence="ECO:0000244|PDB:3Q13" TURN 217..219 /evidence="ECO:0000244|PDB:3Q13" TURN 226..228 /evidence="ECO:0000244|PDB:3Q13" STRAND 234..239 /evidence="ECO:0000244|PDB:3Q13" HELIX 254..262 /evidence="ECO:0000244|PDB:3Q13" HELIX 266..273 /evidence="ECO:0000244|PDB:3Q13" HELIX 274..278 /evidence="ECO:0000244|PDB:3Q13" STRAND 279..285 /evidence="ECO:0000244|PDB:3Q13" STRAND 288..291 /evidence="ECO:0000244|PDB:3Q13" TURN 295..297 /evidence="ECO:0000244|PDB:3Q13" STRAND 302..311 /evidence="ECO:0000244|PDB:3Q13" STRAND 313..322 /evidence="ECO:0000244|PDB:3Q13" STRAND 324..334 /evidence="ECO:0000244|PDB:3Q13" STRAND 340..342 /evidence="ECO:0000244|PDB:3Q13" STRAND 344..350 /evidence="ECO:0000244|PDB:3Q13" STRAND 356..358 /evidence="ECO:0000244|PDB:3Q13" STRAND 401..412 /evidence="ECO:0000244|PDB:3Q13" SEQUENCE 807 AA; 90973 MW; CEA84A83F206A5A5 CRC64; MRLSPAPLKL SRTPALLALA LPLAAALAFS DETLDKVPKS EGYCSRILRA QGTRREGYTE FSLRVEGDPD FYKPGTSYRV TLSAAPPSYF RGFTLIALRE NREGDKEEDH AGTFQIIDEE ETQFMSNCPV AVTESTPRRR TRIQVFWIAP PAGTGCVILK ASIVQKRIIY FQDEGSLTKK LCEQDSTFDG VTDKPILDCC ACGTAKYRLT FYGNWSEKTH PKDYPRRANH WSAIIGGSHS KNYVLWEYGG YASEGVKQVA ELGSPVKMEE EIRQQSDEVL TVIKAKAQWP AWQPLNVRAA PSAEFSVDRT RHLMSFLTMM GPSPDWNVGL SAEDLCTKEC GWVQKVVQDL IPWDAGTDSG VTYESPNKPT IPQEKIRPLT SLDHPQSPFY DPEGGSITQV ARVVIERIAR KGEQCNIVPD NVDDIVADLA PEEKDEDDTP ETCIYSNWSP WSACSSSTCD KGKRMRQRML KAQLDLSVPC PDTQDFQPCM GPGCSDEDGS TCTMSEWITW SPCSISCGMG MRSRERYVKQ FPEDGSVCTL PTEETEKCTV NEECSPSSCL MTEWGEWDEC SATCGMGMKK RHRMIKMNPA DGSMCKAETS QAEKCMMPEC HTIPCLLSPW SEWSDCSVTC GKGMRTRQRM LKSLAELGDC NEDLEQVEKC MLPECPIDCE LTEWSQWSEC NKSCGKGHVI RTRMIQMEPQ FGGAPCPETV QRKKCRIRKC LRNPSIQKLR WREARESRRS EQLKEESEGE QFPGCRMRPW TAWSECTKLC GGGIQERYMT VKKRFKSSQF TSCKDKKEIR ACNVHPC
Research Areas
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Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.


Rose, J. Phd, et al. Mol. Med. (2010) In press :
Attur, M.G., et al. FASEB J. 23(1):79-89(2009)
Tan, K., et al. J. Mol. Biol. 381(5):1213-1223(2008)
Kitagawa, M., et al. Biochem. Biophys. Res. Commun. 349(3):1050-1056(2006)
Pyle-Chenault, R.A., et al. Tumour Biol. 26(5):245-257(2005)

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$ 325.00
$ 109.00
Cat# AP12414a
Availability: In Stock
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