|Application ||IHC-P, WB, E|
|Calculated MW||71836 Da|
|Antigen Region||607-637 aa|
|Other Names||E3 SUMO-protein ligase PIAS1, 632-, DEAD/H box-binding protein 1, Gu-binding protein, GBP, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, PIAS1, DDXBP1|
|Target/Specificity||This PIAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 607-637 amino acids from the C-terminal region of human PIAS1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PIAS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).|
|Cellular Location||Nucleus speckle Nucleus, PML body. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton|
|Tissue Location||Expressed in numerous tissues with highest level in testis.|
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Provided below are standard protocols that you may find useful for product applications.
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Miyauchi, Y., et al., J. Biol. Chem. 277(51):50131-50136 (2002).
Nishida, T., et al., J. Biol. Chem. 277(44):41311-41317 (2002).
Tan, J.A., et al., J. Biol. Chem. 277(19):16993-17001 (2002).
Megidish, T., et al., J. Biol. Chem. 277(10):8255-8259 (2002).
Liu, B., et al., J. Biol. Chem. 276(39):36624-36631 (2001).
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