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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CYP2S1 Antibody (C-term)   

CYP2S1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - CYP2S1 Antibody (C-term) AP12635B
    Confocal immunofluorescent analysis of CYP2S1 Antibody (C-term)(Cat#AP12635b) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • WB - CYP2S1 Antibody (C-term) AP12635B
    Western blot analysis of lysate from BEAS 2B cell line, using CYP2S1 Antibody (C-term)(Cat. #AP12635b). AP12635b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
    detail
  • WB - CYP2S1 Antibody (C-term) AP12635B
    CYP2S1 Antibody (C-term) (Cat. #AP12635b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CYP2S1 antibody detected the CYP2S1 protein (arrow).
    detail
  • IHC-P - CYP2S1 Antibody (C-term) AP12635B
    CYP2S1 Antibody (C-term) (Cat. #AP12635b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2S1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession Q96SQ9
Other Accession NP_085125.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55817 Da
Antigen Region 399-428 aa
Additional Information
Gene ID 29785
Other Names Cytochrome P450 2S1, CYPIIS1, CYP2S1
Target/Specificity This CYP2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-428 amino acids from the C-terminal region of human CYP2S1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP2S1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP2S1 {ECO:0000303|PubMed:11181079, ECO:0000312|HGNC:HGNC:15654}
Function A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids (PubMed:12711469, PubMed:21068195). In epidermis, may contribute to the oxidative metabolism of all-trans- retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:12711469). Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs) (PubMed:21068195). Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage (PubMed:21068195).
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Tissue Location Expressed at higher levels in extrahepatic tissues including trachea, lung, stomach, small intestine, colon, kidney, breast, placenta and spleen (PubMed:11181079, PubMed:12711469) Expressed in peripheral blood leukocytes (PubMed:11181079) Constitutively expressed in skin (at protein level) (PubMed:12711469)
Research Areas
Citations ( 0 )

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined.

References

Bui, P.H., et al. Mol. Pharmacol. 76(5):1031-1043(2009) Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009) Deb, S., et al. Expert Opin Drug Metab Toxicol 5(4):367-380(2009) Jang, Y.J., et al. Ther Drug Monit 29(3):292-298(2007) Hanzawa, Y., et al. Drug Metab. Pharmacokinet. 22(2):136-140(2007)

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$ 365.00
$ 140.00
Cat# AP12635B
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