|Application ||WB, E|
|Other Accession||Q5M7V1, Q9CYB0, NP_005789.2, NP_434698.1, NP_001007279.1, NP_998755.1|
|Calculated MW||46988 Da|
|Antigen Region||61-90 aa|
|Other Names||E3 ubiquitin-protein ligase TRIM13, 632-, B-cell chronic lymphocytic leukemia tumor suppressor Leu5, Leukemia-associated protein 5, Putative tumor suppressor RFP2, RING finger protein 77, Ret finger protein 2, Tripartite motif-containing protein 13, TRIM13, LEU5, RFP2, RNF77|
|Target/Specificity||This TRIM13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human TRIM13.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRIM13 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||LEU5, RFP2, RNF77|
|Function||Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor (PubMed:22178386). Plays also a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation (PubMed:28087809). Participates as well in T-cell receptor- mediated NF-kappa-B activation (PubMed:25088585). In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B (PubMed:25152375).|
|Cellular Location||Endoplasmic reticulum membrane; Single- pass membrane protein Note=Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia. Multiple alternatively spliced transcript variants have been found for this gene.
Lerner, M., et al. Mol. Biol. Cell 18(5):1670-1682(2007)
Skoblov, M., et al. Biochem. Biophys. Res. Commun. 342(3):859-866(2006)
Corcoran, M.M., et al. Genes Chromosomes Cancer 40(4):285-297(2004)
Dunham, A., et al. Nature 428(6982):522-528(2004)
van Everdink, W.J., et al. Cancer Genet. Cytogenet. 146(1):48-57(2003)
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