EIF4ENIF1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9NRA8 |
Other Accession | NP_062817.2, NP_001157974.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 108201 Da |
Antigen Region | 80-108 aa |
Gene ID | 56478 |
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Other Names | Eukaryotic translation initiation factor 4E transporter, 4E-T, eIF4E transporter, Eukaryotic translation initiation factor 4E nuclear import factor 1, EIF4ENIF1 |
Target/Specificity | This EIF4ENIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the N-terminal region of human EIF4ENIF1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | EIF4ENIF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EIF4ENIF1 (HGNC:16687) |
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Function | EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:27342281, PubMed:32354837). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:24335285, PubMed:32354837). Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation (PubMed:27342281, PubMed:32354837). Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity). Promotes miRNA-mediated translational repression (PubMed:24335285, PubMed:27342281, PubMed:28487484). Required for the formation of P- bodies (PubMed:16157702, PubMed:22966201, PubMed:27342281, PubMed:32354837). Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay (PubMed:32354837). Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism (PubMed:10856257, PubMed:28216671). |
Cellular Location | Cytoplasm, P-body. Cytoplasm. Nucleus. Nucleus, PML body. Nucleus speckle. Note=Predominantly cytoplasmic (PubMed:10856257). Mainly localizes to processing bodies (P-bodies) (PubMed:16157702). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner (PubMed:10856257). Localization to nuclear foci and speckles requires active transcription (PubMed:22090346) |
Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
References
Suzuki, Y., et al. J. Biol. Chem. 284(51):35597-35604(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Lee, H.C., et al. Biochem. Biophys. Res. Commun. 369(4):1160-1165(2008)
Denoeud, F., et al. Genome Res. 17(6):746-759(2007)
Lim, J., et al. Cell 125(4):801-814(2006)
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