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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SH2B3 Antibody (N-Term)   

SH2B3 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - SH2B3 Antibody (N-Term) AP22321a
    Overlay histogram showing Hela cells stained with AP22321a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22321a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - SH2B3 Antibody (N-Term) AP22321a
    Anti-SH2B3 Antibody (N-Term) at 1:2000 dilution + U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9UQQ2
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 63225 Da
Additional Information
Gene ID 10019
Other Names SH2B adapter protein 3, Lymphocyte adapter protein, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk, SH2B3, LNK
Target/Specificity This SH2B3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 67-101 amino acids from the human region of human SH2B3.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSH2B3 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SH2B3
Synonyms LNK
Function Links T-cell receptor activation signal to phospholipase C- gamma-1, GRB2 and phosphatidylinositol 3-kinase.
Tissue Location Preferentially expressed by lymphoid cell lines.
Research Areas
Citations (0)

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Background

Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.

References

Li Y.,et al.J. Immunol. 164:5199-5206(2000).
Bartholomew M.A.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Todd J.A.,et al.Nat. Genet. 39:857-864(2007).
Hunt K.A.,et al.Nat. Genet. 40:395-402(2008).

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$ 109.00
Cat# AP22321a
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