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ANO5 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ANO5 Antibody (C-term) AP8580B
    All lanes : Anti-ANO5 Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 107 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ANO5 Antibody (C-term) AP8580B
    Western blot analysis of ANO5 Antibody (C-term) (Cat. #AP8580b) in mouse spleen tissue lysates (35ug/lane). ANO5 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ANO5 Antibody (C-term) AP8580B
    ANO5 Antibody (C-term) (Cat. #AP8580b) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of theANO5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q75V66
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 107188 Da
Antigen Region 792-819 aa
Additional Information
Gene ID 203859
Other Names Anoctamin-5, Gnathodiaphyseal dysplasia 1 protein, Transmembrane protein 16E, ANO5, GDD1, TMEM16E
Target/Specificity This ANO5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 792-819 amino acids from the C-terminal region of human ANO5.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANO5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANO5
Synonyms GDD1, TMEM16E
Function Does not exhibit calcium-activated chloride channel (CaCC) activity.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Colocalized with CALR/calreticulin (PubMed:15124103). Shows an intracellular localization according to PubMed:22075693.
Tissue Location Highly expressed in brain, heart, kidney, lung, and skeletal muscle. Weakly expressed in bone marrow, fetal liver, placenta, spleen, thymus, osteoblasts and periodontal ligament cells
Citations ( 0 )

Background

ANO5 is a member of the anoctamin family of transmembrane proteins. This protein is likely a calcium activated chloride channel.

References

Katoh,M. et.al., Am. J. Hum. Genet. 75 (5), 927-928 (2004)
Tsutsumi,S., et.al., Am. J. Hum. Genet. 74 (6), 1255-1261 (2004)

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$ 365.00
$ 140.00
Cat# AP8580B
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