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DNM3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DNM3 Antibody (Center) AP8833C
    Western blot analysis of DNM3 Antibody (Center) (Cat. #AP8833c) in HL-60 cell line lysates (35ug/lane). DNM3 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - DNM3 Antibody (Center) AP8833C
    Formalin-fixed and paraffin-embedded human brain tissue reacted with DNM3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - DNM3 Antibody (Center) AP8833C
    DNM3 Antibody (Center) (Cat. #AP8833c) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9UQ16
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 97746 Da
Antigen Region 557-584 aa
Additional Information
Gene ID 26052
Other Names Dynamin-3, Dynamin, testicular, T-dynamin, DNM3, KIAA0820
Target/Specificity This DNM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 557-584 amino acids from the Central region of human DNM3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNM3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNM3
Synonyms KIAA0820
Function Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Research Areas
Citations (0)
citation

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Background

Members of the dynamin family, such as DNM3, possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding.

References

Orth,J.D. et.al., Curr. Opin. Cell Biol. 15 (1), 31-39 (2003)

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$ 182.50
$ 70.00
Cat# AP8833C
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