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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   p33 ING1 Antibody   

p33 ING1 Antibody

     
  • ICC/IF - p33 ING1 Antibody ASM10398
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
  • WB - p33 ING1 Antibody ASM10398
    Western blot analysis of Mouse brain cell lysates showing detection of ING1 protein using Rabbit Anti-ING1 Polyclonal Antibody (ASM10398). Primary Antibody: Rabbit Anti-ING1 Polyclonal Antibody (ASM10398) at 1:1000. Left: cell expressed flag-tag. Right: Untagged.
  • ICC/IF - p33 ING1 Antibody ASM10398
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p33 ING1 Polyclonal Antibody (ASM10398) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9QXV3
Other Accession NP_036049.2
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Description Rabbit Anti-Human p33 ING1 Polyclonal
Target/Specificity Detects ~33kDa.
Other Names Growth inhibitor ING1 Antibody, ING1 Antibody, inhibitor of growth 1 Antibody, p24ING1c Antibody, p33 Antibody, p33ING Antibody, p33ING1b Antibody, p33ING1c Antibody, p47 Antibody, p47ING1a Antibody, tumor suppressor ING1 Antibody
Immunogen Mouse p33 ING1 N-terminal peptide –KLH conjugates
Purification Protein A Purified
Storage -20ºC
Storage Buffer TBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.25 mg/ml was sufficient for detection of SPC-145 in lysates prepared from human melanoma cell lines by western blot analysis.
Cellular Localization Nucleus
Research Areas
Citations (0)

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Background

The p33ING1 protein encodes a 33-kD, 294-amino acid protein that displays the characteristics of a tumor suppressor gene. The p33ING1 protein is a regulator of cell cycle, senescence, and apoptosis. It can mediate growth arrest, anchor dependent growth and influence chemo sensitivity (1-4). It also has a proposed role as a growth regulator, which is consistent with its location in the nucleus (5). It has been demonstrated that the ING1 gene has three exons and that four mRNA variants are transcribed from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 IG1) (6, 7). ING1 can possibly influence the above biological functions depending on which variant is expressed (1-4).

References

1. Cheung, K.J., Jr. and Li, G. (2002) Int. J. Oncol. 21(6): 1361-1365.
2. Cheung, K.J., Jr. and Li, G. (2002) Exp. Cell Res. 279(2): 291-28.
3. Cheung, K.J., Jr. and Li, G. (2002) Int. J. Oncol. 20(6):1319-1322.
4. Cheung, K.J., Jr. and Li, G. (2001) Exp. Cell Res. 268(1): 1-6.
5. Garkavtsev, I. et al. (1996). Nat Genet. 14(4): 415-420.
6. Gunduz, M. et al. (2000). Cancer Res. 60(12): 3143-6.
7. Jager, D. et al. (1999). Cancer Res. 59: 6197-6204.

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Cat# ASM10398
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