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Phosphoserine Antibody: HRP

     
  • ICC/IF - Phosphoserine Antibody: HRP ASM10403
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphoserine Polyclonal Antibody (ASM10403). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody (ASM10403) at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.
  • WB - Phosphoserine Antibody: HRP ASM10403
    Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody (ASM10403). Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody (ASM10403) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1 µM okadaic acid).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF
Host Rabbit
Reactivity Species Independent
Clonality Polyclonal
Description Rabbit Anti-Phosphoserine Polyclonal
Target/Specificity Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Other Names Phospho-ser Antibody, pS Antibody, pSer Antibody, Phospho-serine antibody
Immunogen Phosphoserine conjugated to KLH, and phosvitin mixture
Purification Protein A Purified
Storage 4ºC
Storage Buffer HRP Stabilizing buffer
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 2 µg/ml of SPC-151 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 µM okadaic acid.
Citations (0)

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Background

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).

References

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.
2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.
3. Downward J. (2001) Nature 411: 759-762.
4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.
5. Ostrovsky P.C. (1995) Genes Dev. 9(16): 2034-2041.

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Cat# ASM10403
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