Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Phosphoserine Antibody: HRP
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IP, ICC |
|---|---|
| Host | Rabbit |
| Reactivity | Species Independent |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Phosphoserine Polyclonal |
| Target/Specificity | Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. |
| Other Names | Phospho-ser Antibody, pS Antibody, pSer Antibody, Phospho-serine antibody |
| Immunogen | Phosphoserine conjugated to KLH, and phosvitin mixture |
| Purification | Protein A Purified |
| Storage | 4ºC |
| Storage Buffer | HRP Stabilizing buffer |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 2 µg/ml of SPC-151 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 µM okadaic acid. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
References
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.
2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.
3. Downward J. (2001) Nature 411: 759-762.
4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.
5. Ostrovsky P.C. (1995) Genes Dev. 9(16): 2034-2041.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Shipping Information
