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Methylated Lysine Antibody: Biotin

     
  • ICC/IF - Methylated Lysine Antibody: Biotin ASM10410
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Methylated Lysine Polyclonal Antibody (ASM10410). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (ASM10410) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite.
  • WB - Methylated Lysine Antibody: Biotin ASM10410
    Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (ASM10410). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (ASM10410) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, ICC/IF
Host Rabbit
Reactivity Species Independent
Clonality Polyclonal
Description Rabbit Anti-Methylated Lysine Polyclonal
Target/Specificity Detects proteins containing methylated lysine residues.
Other Names Dimethylysine Antibody, Methyl lysine Antibody, N epsilon dimethyl lysine Antibody, Trimethyl lysine Antibody
Immunogen Methylated KLH Conjugated
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.2-0.5 µg/ml of SPC-159 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.
Citations (0)

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Background

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).

References

1. Yang X.J. (2005) Oncogene. 24:1653-1662.
2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308.
3. Yang X.J. (2004) Bioessays 26:1076-1087.
4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143.
5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218.
6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674.
7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.

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Cat# ASM10410
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