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Phosphotyrosine Antibody

     
  • ICC/IF - Phosphotyrosine Antibody ASM10412
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (ASM10412). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (ASM10412) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite.
  • ICC/IF - Phosphotyrosine Antibody ASM10412
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (ASM10412). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (ASM10412) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite.
  • IHC - Phosphotyrosine Antibody ASM10412
    Immunohistochemistry analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (ASM10412). Tissue: breast cancer. Species: Human . Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (ASM10412) at 1:100.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, ICC/IF
Host Rabbit
Reactivity Species Independent
Clonality Polyclonal
Description Rabbit Anti-Phosphotyrosine Polyclonal
Target/Specificity Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
Other Names Phospho-tyrosine Antibody
Immunogen Phosphotyrosine conjugated to KLH
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.0, 0.01% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:250 dilution of SPC-161 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.
Citations (0)

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Background

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).

References

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.
2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.
3. Downward J. (2001) Nature 411: 759-762.
4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.
5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352.
6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656.
7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.

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Cat# ASM10412
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