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BIM/BOD (IN2) Antibody

     
  • ICC/IF - BIM/BOD (IN2) Antibody ASM10415
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BIM Polyclonal Antibody (ASM10415). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (ASM10415) at 1:100.
  • WB - BIM/BOD (IN2) Antibody ASM10415
    Western blot analysis of Human A549 cell lysates showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (ASM10415). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (ASM10415) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession O43521
Other Accession NP_619527.1
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human BIM/BOD (IN2) Polyclonal
Target/Specificity Detects ~23kDa.
Other Names BCL2 like protein 11 Antibody, Bcl2 interacting mediator of cell death Antibody, Bcl2 interacting protein Bim Antibody, BCL@ like 11 Antibody, BAM Antibody, BimEl Antibody, BimL Antibody, BOD Antibody, BIM beta 6 Antibody, BIM Beta7 Antibody, BIM alpha 3 Antibody, BIM alpha4 Antibody, BIM alpha5 Antibody, BIM alpha6 Antibody
Immunogen Internal central amino acids of human Bim
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 2.5 µg/ml of SPC-164 was sufficient for detection of Bim in 20 µg of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion | Endomembrane System
Research Areas
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Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).

References

1. O'Connor L., Strasser A., O'Reilly L.A., et al. (1998) EMBO J. 17:384-395.
2. Hsu S.Y., Lin P., and Hsueh A.J. (1998) Mol. Endocrinol. 12:1432-40.

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Cat# ASM10415
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