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PUMA (NT) Antibody

  • IHC - PUMA (NT) Antibody ASM10417
    Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (ASM10417). Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10417) at 1:100.
  • WB - PUMA (NT) Antibody ASM10417
    Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (ASM10417). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10417) at 1:500.
  • IHC - PUMA (NT) Antibody ASM10417
    Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (ASM10417). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (ASM10417) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BXH2
Other Accession NP_001120712.1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Description Rabbit Anti-Human PUMA (NT) Polyclonal
Target/Specificity Detects the N-terminal domain of PUMA ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
Other Names BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Immunogen N-terminal amino acids of human PUMA
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 0.02% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 2 µg/ml of SPC-165 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion
Citations (0)

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Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.


1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94.
2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82.
3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.

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Cat# ASM10417
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